GSM3830052: 8h-2h Meki clone c IP; Mus musculus; ChIP-Seq - SRA

SRX5927412: GSM3830052: 8h-2h Meki clone c IP; Mus musculus; ChIP-Seq
1 ILLUMINA (NextSeq 500) run: 57.5M spots, 4.3G bases, 1.6Gb downloads

Submitted by: NCBI (GEO)

Study: Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation [RBP1 ChIP-seq]

PRJNA545337 • SRP199787 • All experiments • All runs

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Extensive analysis if the means by which ERK affects transcription via enhancer regulation in mouse embryonic stem cells. This study combines analysis of mRNA, and nascent RNA dynamics and correlates this with changes in transcription factor, co-factor, and RNAPII binding and activity. Overall design: Mouse embryonic stem cells, stably expressing a cRAF-ErT2 expression cassette, were treated with 4OHT to activate ERK signalling via cRAF-ErT2 for either 2hrs, 8hrs, or 8hrs followed by a subsequent 2hr treatement with the MEK inhibitor PD0325901.

Sample: 8h-2h Meki clone c IP

SAMN11885930 • SRS4840996 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: Cells we fixed in 1% formaldehyde at RT for 10 minutes, nuclei extracted and chromatin sonicated using a bioruptor (10 cycles, 30s on 30s off). Cleared chromatin was incubated with anti-RBP1 antibody (Active Motif, 39097). Input DNA serves as negative control for antibody enrichment. Libraries were prepared using the NEB Next Ultra 2 kit.

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 57.5M spots, 4.3G bases, 1.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR9154332	57,485,904	4.3G	1.6Gb	2019-08-08

ID:: 7955993

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