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SRX5444375: GSM3635617: miR-138_293T3; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 62.9M spots, 6.3G bases, 2.7Gb downloads

Submitted by: NCBI (GEO)
Study: Identification of targets of miR-138 in Neuro-2a and 293T cells by RNAseq
show Abstracthide Abstract
We previously showed that miR-138 can repress herpes simplex virus 1 (HSV-1) ICP0 expression by binding to ICP0 mRNA. However, in this study we found that miR-138 can also repress viral gene expression independent of ICP0. We did not find other confirmed viral targets of miR-138. Therefore we conducted these RNAseq experiments (in combination with PAR-CLIP experiments whose results are uploaded separately) to identify host targets of miR-138 in two cell lines to explain the ICP0-independent effects on HSV-1 gene expression. Overall design: We transfected Neuro-2a cells or 293T cells with a miR-138 mimic or a scrambled control mimic, and compared miR-138 transfected with control transfected cells. Three biological replicates for each condition.
Sample: miR-138_293T3
SAMN11037846 • SRS4421512 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNAs were purified using an RNeasyPlus Mini Kit following the manufacturer's instructions (Qiagen) mRNAs were isolated using a Takara mRNA Isolation Kit. Libraries were prepared using a PrepX RNA-seq Library Preparation Kit
Experiment attributes:
GEO Accession: GSM3635617
Links:
Runs: 1 run, 62.9M spots, 6.3G bases, 2.7Gb
Run# of Spots# of BasesSizePublished
SRR864632762,865,7206.3G2.7Gb2019-03-02

ID:
7360725

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