SRX5328009: ChIP-Seq of Zea mays: EREB_TF106 Zm00001d020267 replicate 2
1 ILLUMINA (HiSeq X Ten) run: 40.9M spots, 12.3G bases, 5Gb downloads
Design: Transcription factor was transciently expressed in maize protoplasts with epitope tag (Avitag or HA). The protoplasts were fixed with 1% formaldehyde for 10 minutes, and lysed with lysis buffer (0.25 M Sucrose in TE with 1% Triton X-100). The chromatin was sonicated and incubated with streptavidine or Protein A dynabeads with anti-HA Ab. The biotin samples were washed 5 times with binding buffer (1 M NaCl, TE, 0.2% Tween20), twice with TE with 0.1% SDS and twice with TE with 0.1% Tween. The HA samples were washed with low salt buffer (0.15 M NaCl, TE, 1% TritonX100), high salt buffer (0.5 M NaCl, TE, 1% TritonX100) and lithium buffer (0.25 M LiCl, TE, 1% TritonX100). After the washing steps, on-bead Tn5 or TruSeq library prep was performed.
Submitted by: The Chinese University of Hongkong
Study:
maize cistromeshow Abstracthide AbstractHigh-throughput mapping of the in-vivo binding preferences of 104 maize transcription factor using ChIP-Seq
Library:
Name: EREB_TF106_rep2
Instrument: HiSeq X Ten
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Runs:
1 run, 40.9M spots, 12.3G bases, 5Gb