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SRX5226959: GSM3554071: Nintedanib_2uM_IPF; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 39M spots, 11.2G bases, 3.6Gb downloads

Submitted by: NCBI (GEO)
Study: Gene Expression Changes Associated with Nintedanib Treatment in Idiopathic Pulmonary Fibrosis Fibroblasts [RNA-seq]
show Abstracthide Abstract
We found strong protein-protein interactions within these dysregulated genes in nintedanib treated IPF fibroblast, with most genes involved in the pathways of cell cycle, mitotic cell cycle, and cell division. In IPF fibroblasts, we found nintedanib treatment was associated with downregulation of has-miR-92a-1-5p, which might de-repress SLC25A23 expression, and upregulation of has-miR-486-5p, which might repress DDX11, E2F1, and PLXNA4 expressions. Overall design: Next-generation sequencing for mRNA and small RNA of IPF fibroblasts treated with nintedanib 2 µM and 4 µM normal and without nintedanib
Sample: Nintedanib_2uM_IPF
SAMN10713582 • SRS4230811 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: total RNAs from Ctrl_IPF fibroblasts, Nintedanib_2uM_IPF fibroblasts and Nintedanib_4uM_IPF fibroblasts were extracted by Trizol Reagent according to the manufacturer's instructions. All RNA Sample preparation procedures were carried out according to the official Illumina protocol. Agilent's SureSelect Strand-Specific RNA Library Preparation Kitwere used for library construction followed by AMPure XP Beads size selection. The sequence was directly determined using Illumina's sequencing-by-synthesis (SBS) technology. Sequencing data (FASTQ files) were generated by Welgene's pipeline based on Illumina's basecalling program bcl2fastq v2.2.0.
Experiment attributes:
GEO Accession: GSM3554071
Links:
Runs: 1 run, 39M spots, 11.2G bases, 3.6Gb
Run# of Spots# of BasesSizePublished
SRR841787439,026,89511.2G3.6Gb2019-04-09

ID:
7063005

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