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SRX5139571: GSM3516948: Patient derived xenograft 332; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 309.4M spots, 30.3G bases, 12.1Gb downloads

Submitted by: NCBI (GEO)
Study: Single cell RNA-seq of 25 thousand epithelial cells from xenografts of triple-negative breast cancers
show Abstracthide Abstract
Patient derived xenografts (PDX) were created from two triple-negative breast cancers (PDX-110 and PDX-332) taken at the time of surgery from drug-naive patients. Freshly sorted epithelial cells were profiled by single-cell RNA-seq (scRNA-seq) using a 10X Genomics Chromium System. Overall design: Transcriptional profiling was completed for 10,060 total epithelial cells from PDX-110 and 14,681 total epithelial cells from PDX-322.
Sample: Patient derived xenograft 322
SAMN10601952 • SRS4151081 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Epithelial cells were isolated by FACS from each PDX. Freshly sorted cells were submitted to a 10X Genomics Chromium System for single cell capture. cDNA synthesis and library preparation was done according to the protocol supplied by the manufacturer. Sequencing was carried out on Illumina NextSeq 500 platform using sequencing parameters recommended for Single Cell 3' V2.0 libraries.
Links:
Runs: 1 run, 309.4M spots, 30.3G bases, 12.1Gb
Run# of Spots# of BasesSizePublished
SRR8327929309,391,82730.3G12.1Gb2019-02-27

ID:
6954013

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