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SRX5007607: GSM3473456: m2_3; Fagopyrum tataricum; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 26M spots, 7.8G bases, 3Gb downloads

Submitted by: NCBI (GEO)
Study: Next Generation Sequencing Facilitates Quantitative Analysis of Seed Development of Easy Dehulling Tartary Buckwheat Transcriptomes
show Abstracthide Abstract
We generated 78.41 Gb of high-quality sequencing data (~8.71 Gb per sample) and catalogued the expression profiles of 9,765 annotated Tartary buckwheat genes in each sample. The analysis showed differences of transcriptomes during the development of easy dehulling buckwheat seed. We identified numerous differentially expressed genes that exhibited distinct expression patterns. These genes have known or potential roles in development of easy dehulling buckwheat seed. Therefore, we are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to easy dehulling character. Overall design: buckwheat seeds mRNA profiles of the development were generated by deep sequencing, in triplicate, using Illumina HiSeq4000.
Sample: m2_3
SAMN10423201 • SRS4041618 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Buckwheat seeds were sampled, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 3 ug of total RNA for the Vonstruction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM3473456
Links:
Runs: 1 run, 26M spots, 7.8G bases, 3Gb
Run# of Spots# of BasesSizePublished
SRR818782226,049,1827.8G3Gb2022-04-01

ID:
6757195

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