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SRX4815768: GSM3422924: Stage E7.5 embryo 3 section 7 region MA (E7.5_3_7MA); Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 4M spots, 995.1M bases, 377.1Mb downloads

Submitted by: NCBI (GEO)
Study: Molecular architecture of lineage specification and tissue organization in early mouse embryo
show Abstracthide Abstract
During postimplantation development of the mouse embryo, descendants of the inner cell mass cells in the early epiblast transit from the naïve pluripotent state to the primed pluripotent state. Concurrent with the transition of the pluripotency states is the specification of cell lineages and formation of germ layers in the embryos that serves as the blueprint for embryogenesis. Fate mapping and lineage analysis studies have revealed that cells in different regions of the germ layers acquire location-specific cell fates during gastrulation. The regionalization of cell fates heralding the formation of the basic body plan is conserved in vertebrate embryos at a common phylotypic stage of development. Knowledge of the molecular regulation that underpin the lineage specification and tissue patterning is instrumental for understanding embryonic programming and stem cell-based translational study. However, a genome-wide molecular annotation of lineage segregation and tissue architecture of post-implantation embryo has yet to be undertaken. Here, we reported a spatially resolved transcriptome of cell populations at defined positions in the germ layers over the period of pre- to late gastrulation development. This spatio-temporal transcriptome provides high resolution digitized gene expression profiles and defines the molecular attribute of the genealogy of lineages and continuum of pluripotency states in time and space. The transcriptome further identifies the networks of molecular determinants that drive lineage specification and tissue patterning in the early postimplantation mouse embryo. Overall design: By using spatial transcriptome of Geo-seq, we carried out transcriptome profiling on embryo sections at a high resolution of 20-40 cells per sample. We then constructed a comprehensive spatial transcriptome map from the pre-gastrulation to late-gastrulation embryos that are visualized in a 3D embryonic model based on the sequencing data. Please be aware that the positions for left (L) and right (R) are from mirrored images and should be considered as right and left in real embryo settings. Anterior (A) or Posterior (P) regions do not change.
Sample: Stage E7.5 embryo 3 section 7 region MA (E7.5_3_7MA)
SAMN10220587 • SRS3891072 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: According to Geo-seq (Chen et al., 2017), RNA from LCM samples were extracted by 5M GuSCN (Invitrogen, # 20012-043), then RNA was precipitated by ethanol with the help of RNA carrier. Libraries were constructed using illumina Nextera XT DNA sample preparation kit
Experiment attributes:
GEO Accession: GSM3422924
Links:
Runs: 1 run, 4M spots, 995.1M bases, 377.1Mb
Run# of Spots# of BasesSizePublished
SRR79845703,980,215995.1M377.1Mb2019-08-08

ID:
6515845

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