Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was isolated using the RNeasy Mini kit (Qiagen), and cDNA was synthesized using the SuperScript III First-Strand Synthesis SuperMix Kit (Invitrogen) following manufacturer's instructions. Poly A-selected sequencing library preparation using TruSeq RNA Sample Prep Kit ver.2 (Illumina) following manufacturer's protocol.