Instrument: Illumina HiSeq 2000
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: SINGLE
Construction protocol: Purified DNA was extracted by gentle RNase elution and Qiagen column purification For sequencing libraries we followed Illumina’s protocol. Purification protocol: Following the ChIRP protocol in Chu et al., 2011, biotinylated DNA oligos antisense to the target RNA were added to crosslinked, sonicated chromatin for target hybridization. Biotinylated ChIRP oligos, RNA target, and associated chromatin/DNA were purified on streptavidin-functionalized magnetic beads. For ChIRP-seq, the recovered DNA was extracted from each sample.