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SRX382908: GSM1274256: Hypoxia_6hr (total RNA)_4; Homo sapiens; RNA-Seq
1 ABI_SOLID (AB 5500xl Genetic Analyzer) run: 35.8M spots, 2.7G bases, 1.3Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: RNA-seq analysis of Hypoxia and Normoxia cultured cancer cells
show Abstracthide Abstract
Purpose: To study differential mRNA and precursor miRNA expression under hypoxia exposure in cancer cells. We treated ovarian cancer cell line A2780 under hypoxia condition at different time points. Normoxi acultured cells at corresponding time point were used as controls. Overall design: A2780 cancer cells, cultured under hypoxia and normoxia. Time points tested: 6hr, 48hr, 6days.
Sample: Hypoxia_6hr (total RNA)_4
SAMN02420275 • SRS507043 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: AB 5500xl Genetic Analyzer
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was isolated using miRVANA RNA isolation kit (Lifetechnologies). RNA qulaity was meassured using Agilent Bioanalyzer, with atleast RIN<8. Starting with 3ug of total RNA for each sample, Ribosomal RNA was depleted using Ribominus (Invitrogen/Life Technologies, Carlsbad, CA) following manufacturer's recommendations.  Sequencing libraries were then prepared and barcoded individually using  the SOLiD™ Total RNA-Seq Kit for Whole Transcriptome Libraries (Life Technologies, Inc., Carlsbad, CA) following manufacturer's recommendations. Prepared samples were then pooled and sequenced using the Life Technology 5500xl sequencer using 75 base forward read only.
Experiment attributes:
GEO Accession: GSM1274256
Links:
External link:
Runs: 1 run, 35.8M spots, 2.7G bases, 1.3Gb
Run# of Spots# of BasesSizePublished
SRR103729835,803,9652.7G1.3Gb2016-10-20

ID:
547677

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