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SRX3785034: GSM3039261: Akita replicate 1; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 83.2M spots, 12.8G bases, 5.6Gb downloads

Submitted by: NCBI (GEO)
Study: Droplet based single cell RNA sequencing profiles of a murine model of type-1 diabetes
show Abstracthide Abstract
Diabetic kidney disease remains the leading cause of end-stage renal disease, affecting ~30% of patients with long-standing type 1 and type 2 diabetes, and is characterized by proteinuria and gradual loss of kidney function. There is intense interest in understanding the responses of the many cell types present in the kidney to the diabetic milleu. In this study, we present single cell transcriptomes of renal cortex samples of wild-type and diabetic (C57BL/6-Ins2Akita/J) mice using Drop-Seq. Overall design: Renal cortex samples from wild type and diabetic Akita mice (n=2 per group) underwent dissociation and Drop-seq.
Sample: Akita replicate 1
SAMN08689882 • SRS3038427 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Renal cortex was collected from one harvested kidney and minced with a scalpel then pushed through a 70 micron sieve followed by digestion with collagenase IV for 30 mins at 37 C. Cells were pelleted by centrifugation for 3 mins at 300g, followed by two washes with PBS. Cell suspensions underwent drop-seq according to the protocol of Macosko et al (PMID: 26000488). Cell barcode is the first 12 nt of read 1. UMI is position 13 to 20 of read 1.
Experiment attributes:
GEO Accession: GSM3039261
Links:
Runs: 1 run, 83.2M spots, 12.8G bases, 5.6Gb
Run# of Spots# of BasesSizePublished
SRR682894583,216,24212.8G5.6Gb2018-03-15

ID:
5217613

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