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SRX377483: GSM1264229: Bcells_untreated_ChIPseq_Cebpa; Mus musculus; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 32.6M spots, 1.5G bases, 861.2Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: C/EBPa poises B cells for rapid reprogramming into iPS cells [ChIP-Seq]
show Abstracthide Abstract
C/EBPa induces transdifferentiation of B cells into macrophages at high efficiencies and enhances reprogramming into induced pluripotent stem cells (iPSCs) when co-expressed with Oct4, Sox2, Klf4 and Myc (OSKM). However, how C/EBPa accomplishes these effects is unclear. We now found that transient C/EBPa expression followed by OSKM activation induces a 100 fold increase in iPSC reprogramming efficiency, involving 95% of the cells. During this conversion pluripotency and epithelial-mesenchymal transition genes become dramatically up-regulated and 60% of the cells express Oct4 within 2 days. C/EBPa acts as a pathbreaker since it transiently makes the chromatin of pluripotency genes more accessible to DNase I. It also induces the expression of the dioxygenase Tet2 and promotes its translocation to the nucleus where it binds to regulatory regions of pluripotency genes that become demethylated following OSKM induction. In line with these findings, overexpression of Tet2 enhances OSKM-induced B cell reprogramming. Since the enzyme is also required for efficient C/EBPa-induced immune cell conversion, our data suggest that Tet2 provides a mechanistic link between iPSC reprogramming and B cell transdifferentiation. The rapid iPS reprogramming approach described should help to fully elucidate the process and has potential clinical applications. Overall design: Change in Cebpa genome binding/occupancy, comparing primary B-cells treated with estradiol for 18h to induce C/EBPa to untreated cells.
Sample: Bcells_untreated_ChIPseq_Cebpa
SAMN02402448 • SRS502359 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: ChIP experiments were performed as described previously (van Oevelen et al, 2008, PMID:20956564). DNA libraries of C/EBPa enriched chromatin fragments were prepared using Illumina's reagents and instructions.
Experiment attributes:
GEO Accession: GSM1264229
Links:
External link:
Runs: 1 run, 32.6M spots, 1.5G bases, 861.2Mb
Run# of Spots# of BasesSizePublished
SRR102962532,573,2581.5G861.2Mb2013-11-18

ID:
539379

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