GSM1262393: RNA-seq_WT_hMSC_rep2; Homo sapiens; RNA-Seq - SRA

SRX376714: GSM1262393: RNA-seq_WT_hMSC_rep2; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 5.3M spots, 1.1G bases, 650.1Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: A Werner syndrome stem cell model unveils heterochromatin alterations as a driver of human aging

PRJNA227344 • SRP032942 • All experiments • All runs

show Abstracthide Abstract

Our data throw light upon the effect of WRN deficiency on gene expression and epigenomic modification, which indicates aging-associated changes from both genomic and epigenomic level. Overall design: It was compared between WRN+/+ and WRN-/- in hESCs and hMSCs that the gene expression landscapes and epigenetic modifications(H3K4me3, H3K27me3, H3K9me3 and 5-methylcytosine).

Sample: RNA-seq_WT_hMSC_rep2

SAMN02401739 • SRS501648 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted from the cells using the RNeasy Mini Kit (Invitrogen, #74104) following the manufacturer’s protocol RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM1262393

Links:

External link: GEO Sample GSM1262393

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 5.3M spots, 1.1G bases, 650.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR1028759	5,314,299	1.1G	650.1Mb	2015-05-13

ID:: 538610

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity