GSM2981814: MM.1S; Homo sapiens; RNA-Seq - SRA

SRX3651964: GSM2981814: MM.1S; Homo sapiens; RNA-Seq
1 ION_TORRENT (Ion Torrent Proton) run: 11.8M spots, 1.4G bases, 694.2Mb downloads

Submitted by: NCBI (GEO)

Study: Transcriptomic analysis of multiple myeloma cell lines

PRJNA433025 • SRP132239 • All experiments • All runs

show Abstracthide Abstract

We found that a small molecule inhibitor of PRMT4 inhibited cell growth of a subset of multiple myeloma cell lines. To identify biomarkers that predict the sensitivity of myeloma cells to PRMT4 inhibition, we performed transcriptomic analysis of multiple myeloma cell lines. Overall design: Amplicon sequencing of thirteen multiple myeloma cell lines was performed on the Ion Torrent platform. Steady-state gene expression profile of sensitive cells were compaired with that of insensitive cells.

Sample: MM.1S

SAMN08469693 • SRS2916501 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Ion Torrent Proton

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was isolated and purified from cultured cells using an RNeasy Mini kit (QIAGEN). A total of 10 ng RNA was reverse transcribed using the Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermo Fisher Scientific) following the manufacturer's protocol. cDNA libraries were amplified and barcoded using Ion AmpliSeq Transcriptome Human Gene Expression core panel and Ion Xpress Barcode Adapter (Thermo Fisher Scientific).

Experiment attributes:

GEO Accession: GSM2981814

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 11.8M spots, 1.4G bases, 694.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR6675398	11,834,007	1.4G	694.2Mb	2018-02-07

ID:: 5052149

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