GSM2911315: 0824 Ips D8 Flag DKG; Mus musculus; RNA-Seq - SRA

SRX3527715: GSM2911315: 0824 Ips D8 Flag DKG; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina MiSeq) run: 6.3M spots, 470M bases, 190.8Mb downloads

Submitted by: NCBI (GEO)

Study: TET1 regulates reprogramming with Vitamin C and relatively small molecules

PRJNA428314 • SRP127954 • All experiments • All runs

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We found a small molecule-DKG, relative to Vitamin C, could replace the function of Viamin C during reprogramming. After comparing transcriptome profiling (RNA-seq) of this molecule to vitamin C, it could provide a framework for more mechanism. Overall design: mRNA profiles of day 3 and day8 during reprogramming with DMSO or Vitamin C or DKG infected Flag or Tet1CD virus.

Sample: 0824 Ips D8 Flag DKG

SAMN08287226 • SRS2803382 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA of cells was harvested using Trizol reagent on ice. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. We used standard Illumina protocols for sequencing to construct RNA libraries.

Experiment attributes:

GEO Accession: GSM2911315

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 6.3M spots, 470M bases, 190.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR6436245	6,307,890	470M	190.8Mb	2020-12-28

ID:: 4902642

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