Instrument: NextSeq 500
Strategy: RIP-Seq
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Construction protocol: Total RNAs were extracted from freshly isolated human UCB CD34+ cells and chemically fragmented. m6A marked RNAs were isolated with a specific antibody. RNA libraries were prepared for sequencing using Clontech/Nextera non-stranded protocol. Libraries were sequenced on two NextSeq flowcells in high-output mode with 76 base single-end reads.