GSM2883205: FTOC_PBSDMSO_1; Mus musculus; RNA-Seq - SRA

SRX3461273: GSM2883205: FTOC_PBSDMSO_1; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 117.3M spots, 8.2G bases, 3.5Gb downloads

Submitted by: NCBI (GEO)

Study: Single-cell RNA sequencing resolves cellular heterogeneity throughout embryonic development of the thymus

PRJNA421909 • SRP126497 • All experiments • All runs

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This dataset consists of single-cell RNA-seq (Drop-seq) data from eight days of mouse thymus development. Samples span from embryonic day 12.5, when the thymus coalesces, until birth. They cover the whole thymus, including mesenchyme, endothelium, epithelium, thymocytes, and other lymphocytes. The dataset also includes Drop-seq data on Rag1-/- embryos and on fetal thymic organ cultures (control and a retinoic acid perturbation). Overall design: A single-cell atlas of the developing mouse thymus.

Sample: FTOC_PBSDMSO_1

SAMN08160107 • SRS2749447 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Thymic lobes were mechanically isolated, dissociated in trypsin, depleted of RBC's (only some samples), washed and stained with 7AAD cell viability dye, and 7AAD negative single cells were sorted on a BD FACSARIA. Libraries were prepared according to the Drop-seq lab protocol version 3.1 (Macosko and Goldman, December 2015, McCarroll Lab). Final libraries were sequenced on an Illumina NextSeq 500 using high-output 75-cycle kits.

Experiment attributes:

GEO Accession: GSM2883205

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 117.3M spots, 8.2G bases, 3.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR6365754	117,341,885	8.2G	3.5Gb	2018-06-05

ID:: 4821287

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