GSM2874043: Biotin positive 3; Mus musculus; RNA-Seq - SRA

SRX3440421: GSM2874043: Biotin positive 3; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 24.1M spots, 1.8G bases, 717.1Mb downloads

Submitted by: NCBI (GEO)

Study: RNA sequencing of dendritic cells undergoing interaction with T cells in vivo

PRJNA420958 • SRP126061 • All experiments • All runs

show Abstracthide Abstract

We used RNA sequencing to characterize gene expression of dendritic cells from mouse lymph node that, based on LIPSTIC labeling, underwent interaction with CD4+ T cells. Overall design: Antigen pulsed dendritic cells (DCs) were transferred into recipient mice, followed by antigen specific CD4+ T cells. Forty-eight hours after T cell transfer, endogenous dendritic cells were isolated by facs sorting from mouse lymph node and analyzed based on their in vivo LIPSTIC labeling.

Sample: Biotin positive 3

SAMN08127190 • SRS2731266 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Fresh cells were sorted (150 cells/sample) directly into plates containing TCL buffer (Qiagen) supplemented with 1% β-mercaptoethanol using a FACS Aria II (BD Biosciences). RNA from sorted populations was isolated using Agencourt RNAClean XP beads (Beckman Coulter). Full-length cDNA and sequencing libraries were prepared using the Smart-seq2 protocol as described in Picelli et al., 2014. Libraries were sequenced on a Nextseq500 (Illumina) to generate 38 base pair, paired-end reads.

Experiment attributes:

GEO Accession: GSM2874043

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 24.1M spots, 1.8G bases, 717.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR6342201	24,084,111	1.8G	717.1Mb	2018-01-02

ID:: 4797303

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