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SRX3437585: GSM2872477: H3K4me1_CT_+RA; Homo sapiens; ChIP-Seq
2 ILLUMINA (Illumina HiSeq 1500) runs: 59.3M spots, 3G bases, 1.6Gb downloads

Submitted by: NCBI (GEO)
Study: Genomic occurrence analysis of PRMT6-dependent H3R2me2a
show Abstracthide Abstract
Protein arginine methyltransferase 6 (PRMT6) is an epigenetic regulator of fundamental cellular processes, such as gene expression and DNA repair. Asymmetric dimethylation of histone H3 at arginine 2 (H3R2me2a) is the major histone modification catalyzed by PRMT6. To identify the genome-wide deposition and transcriptional impact of H3R2me2a, we established PRMT6 deletion in a human cell model of neural differentiation. These knockout cells show severe neural differentiation defects. ChIP-seq profiling reveals that H3R2me2a is present at promoter as well as non-promoter sites in a PRMT6-dependent manner. Loss of H3R2me2a causes enhanced H3K4me3 deposition and target gene transcription supporting a genome-wide repressive nature of H3R2me2a. Intriguingly, the non-promoter H3R2me2a peaks co-localize with active enhancer marks, such as H3K4me1 and H3K27ac. Overall design: Examining of PRMT6-dependent H3R2me2a deposition in CRISPR control and knockout PRMT6 with neighboring histone modification.
Sample: H3K4me1_CT_+RA
SAMN08120273 • SRS2728530 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 1500
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Cells were crosslinked in the presence of 1% formaldehyde. Cell lysates were sonicated and Protein-DNA complexes were enriched by immunoprecipitation with mentioned antibodies. Libraries were prepared according to the manufacturer's protocol (Diagenode, MicroPlex Library Preparation Kit).
Experiment attributes:
GEO Accession: GSM2872477
Links:
Runs: 2 runs, 59.3M spots, 3G bases, 1.6Gb
Run# of Spots# of BasesSizePublished
SRR633863229,563,1311.5G826.2Mb2018-09-27
SRR633863329,774,7381.5G832.1Mb2018-09-27

ID:
4790019

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