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SRX3344844: GSM2836566: Beko_GSI_rep2; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 1500) run: 52M spots, 2.7G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)
Study: Differential Notch target genes expression profile upon HDAC3 loss-of-function.
show Abstracthide Abstract
The Notch signaling pathway regulates several differentiation and developmental processes in both pre- and post-natal life and its aberrant regulation leads to diseases, including cancer. Here, we investigated the role of histone deacetylase 3 (HDAC3) on the regulation of the Notch-dependent gene expression program in mouse pre-T (Beko) cells. We first defined Notch target genes as those genes that are downregulated upon inhibition of the Notch pathway using DAPT, a gamma-secretase inhibitor (GSI). Subsequently, we investigated the role of HDAC3 in the regulation of Notch target genes by shRNA- and pharmacologically (apicidin)-mediated loss-of-function of HDAC3. Overall design: Mouse pre-T cells (Beko) were treated with gamma-secretase inhibitor (GSI) DAPT to identify Notch target genes by RNA-seq analysis. The function of HDAC3 at Notch target genes was investigated by shRNA- or pharmacological (Apicidin)-mediated loss-of-function of HDAC3 followed by RNA-seq analysis.
Sample: Beko_GSI_rep2
SAMN07957918 • SRS2647918 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 1500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Rneasy Mini Kit (Qiagen #74104) using the QIAshredder (Qiagen #79654) and RNase-free DNase (Qiagen #79254). Lybrary-prep kit: Illumina TruSeq® Stranded Total RNA LT - Ribo-Zero Gold (RS-122-2301/2).
Experiment attributes:
GEO Accession: GSM2836566
Links:
Runs: 1 run, 52M spots, 2.7G bases, 1.3Gb
Run# of Spots# of BasesSizePublished
SRR623647852,043,7142.7G1.3Gb2020-02-20

ID:
4674875

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