GSM2832770: JKY690 chrIII tDNAD biological replicate 1; Saccharomyces... - SRA

SRX3334103: GSM2832770: JKY690 chrIII tDNAD biological replicate 1; Saccharomyces cerevisiae; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 31.8M spots, 9.5G bases, 3.3Gb downloads

Submitted by: NCBI (GEO)

Study: Transfer RNA Genes Affect Chromosome Architecture and Function [RNA-seq]

PRJNA415993 • SRP121795 • All experiments • All runs

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In this study, we investigated the role of tranfer RNA genes (tDNAs) in genome organization and nuclear function by generating a chromosome lacking all its tDNAs. Our analyses of this tDNA-less chromosome show that loss of these genes affects nucleosome postioning, SMC protein binding, centromere clustering, long range chromosome folding and epigenetic silencing. Overall design: We compared the transcriptome in wild type and tDNA-less chromosome III strains by RNA Seq.

Sample: JKY690 chrIII tDNAD biological replicate 1

SAMN07839704 • SRS2636522 • All experiments • All runs

Organism: Saccharomyces cerevisiae

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was isolated by ACGT Inc., Wheeling IL using the Qiagen Rneasy kit. Libraries were constructed and sequenced by ACGT Inc., Wheeling IL using the NEXTflex Rapid Directional RNA-Seq kit

Experiment attributes:

GEO Accession: GSM2832770

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 31.8M spots, 9.5G bases, 3.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR6225495	31,753,689	9.5G	3.3Gb	2019-03-06

ID:: 4663232

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