Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: After flow sorting, Ngn3-eGFP+ cells were collected in RPMI medium. We used mouth pipette to pick single cells into PCR tubes with MATQ-seq lysis buffer (Sheng et al., 2017). MATQ-seq was then performed to amplify the whole transcriptome of single cells. After second strand synthesis, the input and yield of each sample was quantified by qPCR. Highly degraded samples or samples with multiple cells were discarded. After PCR amplification, we performed library preparation and data analysis according to the previous publication (Sheng et al., 2017). Duplex specific Nuclease was used to remove the majority of ribosomal cDNA.