GSM2630345: Nalm6_Input; Homo sapiens; ChIP-Seq - SRA

SRX2831681: GSM2630345: Nalm6_Input; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 9.7M spots, 989.7M bases, 627.4Mb downloads

Submitted by: NCBI (GEO)

Study: Common lymphoid progenitor derivation of plasmacytoid dendritic cells is mediated by Bcl11a

PRJNA387006 • SRP107199 • All experiments • All runs

show Abstracthide Abstract

Plasmacytoid dendritic cells (pDC) specialize in type I interferon production to coordinate innate and adaptive immunity. We identify two pDC subsets in vivo, evaluate their genome-wide expression, and demonstrate a developmental requirement for Bcl11a to provide insight into this enigmatic cell type. Overall design: ChIP sequenicng was performed for a transcription factor of BCL11A in the Nalm6 B cell line and the Raji B cell line. Input was sequenced and used as a control.

Sample: Nalm6_Input

SAMN07138114 • SRS2206724 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2000

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: PAIRED

Construction protocol: ChIP was performed by cross-linking proteins to DNA using 1% formaldehyde solution. Immunoprecipitated DNA was sequenced using Illumina sequencers. Pull-downed DNA was applied for generation of sequencing libraries using the NEB ChIP-seq library prep. kit (E6200L ) following by manufacturer’s instructions.

Experiment attributes:

GEO Accession: GSM2630345

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 9.7M spots, 989.7M bases, 627.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR5573015	9,703,058	989.7M	627.4Mb	2017-11-04

ID:: 4067835

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