GSM2598182: SD_Wk4 post-5/6Nx_RNASeq_rep2; Rattus norvegicus; RNA-Seq - SRA

SRX2781689: GSM2598182: SD_Wk4 post-5/6Nx_RNASeq_rep2; Rattus norvegicus; RNA-Seq
8 ILLUMINA (Illumina HiSeq 2000) runs: 31.1M spots, 6.3G bases, 4.1Gb downloads

Submitted by: NCBI (GEO)

Study: Molecular Mediators of Cardiac Pathology in Cardiorenal Syndrome Type 4 [mRNA]

PRJNA385373 • SRP106491 • All experiments • All runs

show Abstracthide Abstract

We report the changes in left ventricle mRNA abundance in response to 5/6 nephrectomy surgery Overall design: Ten week old male Sprague Dawley rats were subjected to the excision model of 5/6 nephrectomy (5/6Nx) or sham surgery. Left ventricle tissue was collected 2, 4, 5 or 7 weeks later for mRNAsequencing.

Sample: SD_Wk4 post-5/6Nx_RNASeq_rep2

SAMN06887362 • SRS2164162 • All experiments • All runs

Organism: Rattus norvegicus

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Left ventricle tissues were isolated and flash frozen in liquid nitrogen. They were then ground into powder under liquid nitrogen which was thawed in Trizol for chloroform-based RNA isolation. mRNA libraries were constructed for these pools using the Illumina TruSeq Stranded Total RNA Kit. Samples were multiplexed seven per lane on a 300 Gb flow cell and paired-end sequenced with an Illumina HiSeq 2000.

Experiment attributes:

GEO Accession: GSM2598182

Links:

NCBI link: NCBI Entrez (gds)

Runs: 8 runs, 31.1M spots, 6.3G bases, 4.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR5504262	4,000,000	808M	540.5Mb	2017-05-07
SRR5504263	4,000,000	808M	546.8Mb	2017-05-07
SRR5504264	4,000,000	808M	547.4Mb	2017-05-07
SRR5504265	4,000,000	808M	550.3Mb	2017-05-07
SRR5504266	4,000,000	808M	533.6Mb	2017-05-07
SRR5504267	4,000,000	808M	539.8Mb	2017-05-07
SRR5504268	4,000,000	808M	539.7Mb	2017-05-07
SRR5504269	3,067,089	619.6M	420.4Mb	2017-05-07

ID:: 4010152

SRA

Sequence Read Archive

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