Instrument: Illumina HiSeq 2500
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: Genomic DNA was extracted with the Quick-gDNA™ MicroPrep (ZymoResearch) kit according to the manufacturer's instruction. Extracted gDNA was digested with DpnI (NEB, R0176S) and DamID adapter were then ligated using T4 DNA Ligase (M0202L). After ligation, the DNA was digested with DpnII (R0543S) and the DamID fragments of interest were amplified with the KAPA HiFi Polymerase (Roche, 07958935001). DNA was then purified using homemade SPRI magnetic beads and 50 ng of DNA were used as input for DamID-seq library preparation by using the Nextera® DNA Sample Preparation Kit (according to manufacturer's instructions). The libraries were quantitated using the The Qubit® 2.0 Fluorometer (Thermo Fisher). The DNA size distribution of the library was measured by an Agilent Bioanalyzer. Each sample was prepared in 3 or 4 biological replicates.