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SRX2754652: GSM2586978: Oct4 ESC 1M 3; Mus musculus; OTHER
2 ILLUMINA (Illumina HiSeq 2500) runs: 58.1M spots, 5.8G bases, 1.9Gb downloads

Submitted by: NCBI (GEO)
Study: Mammalian low cell number and in vivo DamID-seq for transcription factor binding analyses
show Abstracthide Abstract
Mammalian low cell number and in vivo DamID-seq for transcription factor binding analyses Overall design: DamID-seq peaks were called for Oct4 from mouse embryonic stem cells (mESC), late streak, early bud mouse embryos (LSEB) and DamID-seq peaks were called for Sox2 from ES-derived neural stem cells (NSC) DNase-seq hyper-sensitivity data was downloaded from GEO with the BioSample accession number GSM1014154 and compared with DamID-seq signal for E14 mouse embryonic stem cells. This data is linked below as DNase-seq_GSM1014154.tar. ChIP-Seq raw data was downloaded from GEO (GSE11724, GSE44286, GSE56098) and ERA (ERR216111, ERR414096, ERR414097). Peaks called from Oct4 ChIP-seq were compared to Oct4 DamID-seq peaks for E14 mouse embryonic stem cells. This data is linked below as ChIP-Seq_reanalysis.tar.
Sample: Oct4 ESC 1M 3
SAMN06821119 • SRS2139900 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2500
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: Genomic DNA was extracted with the Quick-gDNA™ MicroPrep (ZymoResearch) kit according to the manufacturer's instruction. Extracted gDNA was digested with DpnI (NEB, R0176S) and DamID adapter were then ligated using T4 DNA Ligase (M0202L). After ligation, the DNA was digested with DpnII (R0543S) and the DamID fragments of interest were amplified with the KAPA HiFi Polymerase (Roche, 07958935001). DNA was then purified using homemade SPRI magnetic beads and 50 ng of DNA were used as input for DamID-seq library preparation by using the Nextera® DNA Sample Preparation Kit (according to manufacturer's instructions). The libraries were quantitated using the The Qubit® 2.0 Fluorometer (Thermo Fisher). The DNA size distribution of the library was measured by an Agilent Bioanalyzer. Each sample was prepared in 3 or 4 biological replicates.
Experiment attributes:
GEO Accession: GSM2586978
Links:
Runs: 2 runs, 58.1M spots, 5.8G bases, 1.9Gb
Run# of Spots# of BasesSizePublished
SRR546932529,619,8143G975.1Mb2018-03-13
SRR546932628,528,4582.9G964.1Mb2018-03-13

ID:
3973946

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