GSM2572248: cortex RNA-seq rep1; Mus musculus; RNA-Seq - SRA

SRX2731667: GSM2572248: cortex RNA-seq rep1; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 65.5M spots, 19.7G bases, 8.5Gb downloads

Submitted by: NCBI (GEO)

Study: Whole-genome analysis of 5-hydroxymethylcytosine and 5-methylcytosine at base resolution in the mouse brain

PRJNA382333 • SRP103763 • All experiments • All runs

show Abstracthide Abstract

The study provides new insights into how 5mC/5hmC vary across different part of brain, and the association and distinct roles of 5mC/5hmC in regulating tissue identity and specification. Overall design: Examination of hydroxymethylomes of mouse cortex, olfactory bulb and cerebellum tissues,as well as methylomes and allele-specific transcriptomes of the same tissues

Sample: cortex RNA-seq rep1

SAMN06704540 • SRS2119560 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: The tissues for the technical replicates are from independent mouse, and at least two replicates are included in different assay as indicated. RNA (Life Tech, at#: 15596-018) were extracted according to the manufacturers’ instructions. Libraries were prepared using the NEB Ultra™ Directional RNA Library Prep Kit according to the manufacturer’s protocol and sequenced using an Illumina Hiseq 2000.

Experiment attributes:

GEO Accession: GSM2572248

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 65.5M spots, 19.7G bases, 8.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR5441810	65,543,299	19.7G	8.5Gb	2019-01-03

ID:: 3935879

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