Instrument: Illumina HiSeq 2000
Strategy: MBD-Seq
Source: GENOMIC
Selection: MBD2 protein methyl-CpG binding domain
Layout: SINGLE
Construction protocol: DNA was extracted, RNase treated, and purified (DNeasy, Qiagen). Methylated DNA was captured using methyl-binding domain protein immunoprecipitation (MBD-IP; MethylMiner Kit, Invitrogen) DNA sequencing was performed at Hudson Alpha using NEBNext reagents (New England Biolabs) according to manufacturer's recommendations with minor modifications (including the use of custom library adapters and indexes).