GSM2490586: 0 min No stimulation (4-38); Mus musculus; RNA-Seq - SRA

SRX2562553: GSM2490586: 0 min No stimulation (4-38); Mus musculus; RNA-Seq
1 ILLUMINA (Illumina MiSeq) run: 188,302 spots, 28.2M bases, 12.2Mb downloads

Submitted by: NCBI (GEO)

Study: Measuring signaling and RNA-Seq in the same cell links gene expression to dynamic patterns of NF-?B activation

PRJNA374736 • SRP099680 • All experiments • All runs

show Abstracthide Abstract

We measure translocation dynamics of NF-?B and perform single-cell RNA-sequencing in the same individual cell. We use this data to identify genes that are responsive to the dynamics of NF-?B activation Overall design: Single-cell RNA sequencing libraries (Smart-Seq2) for ~800 RAW 264.7 cells across 10 libraries are given. Cells were stimulated with LPS on a fluidigm C1 chip - data for both stimulated and unstimulated (control) cells are provided.

Sample: 0 min No stimulation (4-38)

SAMN06334009 • SRS1979365 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Lysis was performed inside a microfluidic chip Libraries were prepared according to protocols for Clontech's SMARTer library preparation kit for the fluidigm C1 SMARTer single cell RNA-seq kit for the Fluidigm C1

Experiment attributes:

GEO Accession: GSM2490586

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 188,302 spots, 28.2M bases, 12.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR5257119	188,302	28.2M	12.2Mb	2017-02-16

ID:: 3704945

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