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SRX2551000: GSM2482841: Flutamide+PPC_2; Homo sapiens; RNA-Seq
2 ILLUMINA (Illumina HiSeq 3000) runs: 40.6M spots, 2.4G bases, 862.3Mb downloads

Submitted by: NCBI (GEO)
Study: A combinatorial screen of the CLOUD uncovers a synergy targeting the androgen receptor
show Abstracthide Abstract
Approved drugs are invaluable tools to study biochemical pathways and further characterization of these compounds may lead to repurposing of single drugs or combinations. Here, we describe a collection of 308 small molecules representing the diversity of structures and molecular targets of all FDA-approved chemical entities. The CeMM Library of Unique Drugs (CLOUD) covers prodrugs and active forms at pharmacologically relevant concentrations and is ideally suited for combinatorial studies. We screened pairwise combinations of CLOUD drugs for impairment of cancer cell viability and discovered the synergistic interaction between flutamide and phenprocoumon (PPC). The combination of these two drugs modulates the stability of the androgen receptor (AR) and resensitizes AR mutant prostate cancer cells to flutamide. Mechanistically, we show that the AR is a substrate for ?-carboxylation, a post-translational modification inhibited by PPC. Collectively, our data suggest that PPC might be repurposed to tackle resistance to antiandrogens in prostate cancer patients. Overall design: Two biological replicates were produced for each condition. Drug treatment was compared to vehicle-treated cells.
Sample: Flutamide+PPC_2
SAMN06320332 • SRS1968983 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 3000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Cells were lysed and RNA isolated using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. The amount of total RNA was quantified using Qubit 2.0 Fluorometric Quantitation system and the RNA integrity number (RIN) was determined using Experion Automated Electrophoresis System RNA-Seq libraries were prepared with TruSeq Stranded mRNA LT sample preparation kit using Sciclone and Zephyr liquid handling robotics. Library amount was quantified using the Qubit 2.0 Fluorometric Quantitation system and the size distribution was assessed using Experion Automated Electrophoresis System
Experiment attributes:
GEO Accession: GSM2482841
Links:
Runs: 2 runs, 40.6M spots, 2.4G bases, 862.3Mb
Run# of Spots# of BasesSizePublished
SRR524429620,016,0631.2G428Mb2017-03-09
SRR524429720,596,6911.2G434.3Mb2017-03-09

ID:
3692241

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