U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX2529734: GSM2473149: J1TI1; Mus musculus; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 33.8M spots, 3.4G bases, 1.1Gb downloads

Submitted by: NCBI (GEO)
Study: A family of double-homeodomain transcription factors promotes zygotic genome activation in placental mammals [ChIP-seq_d2]
show Abstracthide Abstract
In metazoans, the fertilized embryo is transcriptionally mostly silent for a few cell divisions, until release of a first major wave of embryonic transcripts by so-called zygotic genome activation (ZGA). Maternally provided ZGA-triggering factors have been identified in Drosophila melanogaster and Danio rerio but their mammalian homologues are still undefined. Here, we reveal that DUX family transcription factors are key to this process in human and mouse. First, human Dux4 and murine Dux are expressed prior to ZGA in both species. Second, both orthologues bind to and activate the promoters of ZGA genes. Third, Dux knockdown hinders the progression of mouse embryos beyond the 2-cell stage. Fourth, it prevents the cycling of mouse embryonic stem (ES) cells through a 2-cell-like state whereas, conversely, Dux overexpression induces the accumulation of these ZGA genes-expressing ES cells. We conclude that DUX proteins are master regulators of mammalian ZGA. Overall design: ChIP-sequencing analysis for endogenous Trim28 in mESCs and for H3K9me3 in mESC depleted of Trim28 or control
Sample: J1TI1
SAMN06284972 • SRS1950659 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2500
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Chromatin was prepared from 10 million cells as previously described (Rowe et al., 2013b). Libraries were ligated with Illumina adaptors
Experiment attributes:
GEO Accession: GSM2473149
Links:
Runs: 1 run, 33.8M spots, 3.4G bases, 1.1Gb
Run# of Spots# of BasesSizePublished
SRR521969733,807,0423.4G1.1Gb2017-03-25

ID:
3660879

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...