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SRX25044392: GSM8321980: A4_Ezh2I_1_S27; Mus musculus; OTHER
1 ILLUMINA (NextSeq 2000) run: 59M spots, 7.6G bases, 2.4Gb downloads

External Id: GSM8321980_r1
Submitted by: Cellular Reprogramming and Ageing, Institute for Molecular Biology, The University of Queensland
Study: Fosl2 overexpression and Ezh2 inhibition in mouse embryonic fibroblasts
show Abstracthide Abstract
To assess the impact of AP-1 chromatin remodelling, we over-expressed one AP-1 family member, namely Fra2 (encoded by Fosl2), in mouse embryonic fibroblasts (MEFs). To assess the impact of general chromatin depression, we inhibited the activity of PRC2 component histone-lysine N-methyltransferase Ezh2 in MEFS. Overall design: Fosl2 overexpression: We transduced mouse embryonic fibroblasts (MEFs) with a CRISPRa-VPR-mCherry construct and assessed more immediate and longer term effects of Fra2 over-expression by performing OMNI-ATACseq on FACS purified mCherry+/THY1+ cells 4 and 11 days following lentiviral infection with Fra2 gRNAs or scrambled control. For Ezh2 inbition: Following three days of treatment with the Ezh2 inhibitor (Sigma-Aldrich, GSK343) at 4microM and a washout day, MEFs were submitted to the ATAC-seq assay.
Sample: A4_Ezh2I_1_S27
SAMN41786541 • SRS21741542 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM8321980
Instrument: NextSeq 2000
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: We transduced mouse embryonic fibroblasts with a CRISPRa-VPR–mCherry construct and assessed more immediate and longer term effects of Fra2 over-expression by performing OMNI-ATACseq on FACS purifed mCherry+/THY1+ cells 4 and 11 days following lentiviral infection with Fra2 gRNAs or scrambled control.For Ezh2 inbition:Following three days of treatment with the Ezh2 inhibitor (Sigma-Aldrich, GSK343) at 4μM and a washout day, MEFs were submitted to the ATAC-seq assay.
Runs: 1 run, 59M spots, 7.6G bases, 2.4Gb
Run# of Spots# of BasesSizePublished
SRR2953648658,952,5887.6G2.4Gb2024-07-15

ID:
33381644

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