GSM8313939: X1i; Rattus norvegicus; RNA-Seq - SRA

SRX24839475: GSM8313939: X1i; Rattus norvegicus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 65.4M spots, 13.2G bases, 3.8Gb downloads

External Id: GSM8313939_r1

Submitted by: Day, Neurobiology, UAB

Study: RNA-seq dataset for "Reelin marks cocaine-activated striatal ensembles, promotes neuronal excitability, and regulates cocaine reward"

PRJNA1121282 • SRP512465 • All experiments • All runs

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This dataset contains RNA-seq results from rat embryonic striatal neuronal cultures treated with CRISPRi machinery (dCas9-KRAB-MeCP2 fusion) with a CRISPR sgRNA targeting lacZ (a bacterial gene not found in the mammalian genome; used as a non-targeting control) or Reln. Overall design: This experiment contains 8 biological samples, each of which underwent RNA-seq on an Illumina NovaSeq6000 instrument. Samples were treated with CRISPRi machinery targeting lacZ (lacZ control; samples X1i, X2i, X3i, X4i), or Reln (samples X5i, X6i, X7i, X8i) . Forward and reverse paired-end reads are denoted as "R1" or "R2" in raw file names.

Sample: X1i

SAMN41748868 • SRS21551028 • All experiments • All runs

Organism: Rattus norvegicus

Library:

Name: GSM8313939

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA from was extracted, DNase-treated, and purified (RNeasy, Qiagen). 1µg of total RNA underwent quality control (Bioanalyzer; all RIN values > 7.0) and library construction for polyA+ RNA sequencing. 1 μg of total RNA underwent quality control (Bioanalyzer) and was prepared for directional RNA sequencing using NEBNext reagents (New England Biolabs) according to manufacturer's recommendations. Specifically, the NEBNext Poly(A) mRNA manetic isolation module was used to enrich polyadenylated RNA. RNA-seq libraries underwent sequencing (100 bp paired-end directional reads) on an Illumina sequencing platform (NovaSeq6000).

Runs: 1 run, 65.4M spots, 13.2G bases, 3.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR29323024	65,382,208	13.2G	3.8Gb	2024-07-10

ID:: 33173809

SRA

Sequence Read Archive

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