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SRX2464640: GSM2446223: Co-culture replicate 1, 13:11 day:night cycle, Time 36; Alteromonas macleodii; Prochlorococcus marinus str. NATL2A; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 2.4M spots, 189.4M bases, 133.5Mb downloads

Submitted by: NCBI (GEO)
Study: What happens to Prochlorococcus when the sun does not come up?
show Abstracthide Abstract
We examine how the transcriptome of Prochlorococcus strain NATL2A changes in response to extended light deprivation, both when grown alone and in the presence of a naturally co-occurring heterotroph, Alteromonas macleodii MIT1002. Overall design: Samples are collected over time from cultures experiencing either a 13:11 light:dark light cycle or that remain in the dark following the end of the expected night. Samples are collected from each of 3 biological replicate cultures of Prochlorococcus, grown either alone or in co-culture with a marine Alteromonas.
Sample: Co-culture replicate 1, 13:11 day:night cycle, Time 36
SAMN06205890 • SRS1897121 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Culture samples added to cold RNAlater at each timepoint, cells collected onto a 0.2µm filter, and total RNA extracted with the mirVana kit (Ambion). Strand-specific RNA-seq library construction starting with 100ng of total RNA using the dUTP second-strand approach; duplex-specific normalization was performed to reduce ribosomal RNA content in each sample.
Experiment attributes:
GEO Accession: GSM2446223
Links:
Runs: 1 run, 2.4M spots, 189.4M bases, 133.5Mb
Run# of Spots# of BasesSizePublished
SRR51465842,367,161189.4M133.5Mb2018-05-01

ID:
3577959

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