GSM2442797: WT_TB_rep3; Mus musculus; RNA-Seq - SRA

SRX2452543: GSM2442797: WT_TB_rep3; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 1500) run: 15.4M spots, 1.6G bases, 898.1Mb downloads

Submitted by: NCBI (GEO)

Study: Defects in dosage compensation impact global gene regulation in the mouse trophoblast

PRJNA359489 • SRP095851 • All experiments • All runs

show Abstracthide Abstract

X chromosome inactivation (XCI) is critical for the development of the extraembryonic tissue as well as the embryonic tissue. Here we carried out transcriptomic analysis of the trophoblast isolated from two different Xist mutant embyros at E7.5 in comparison with wild-type. Overall design: Allele-specific RNA-seq analysis of the trophoblast isolated from E7.5 embryos. Embryos were obtained from JF1 females crossed with B6 males carrying respective Xist mutations as well as B6 wild-type males.

Sample: WT_TB_rep3

SAMN06192127 • SRS1885905 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 1500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Trophoblasts were isolated from E7.5 embryos. RNA was prepared using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#15027078) was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM2442797

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 15.4M spots, 1.6G bases, 898.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR5136513	15,430,214	1.6G	898.1Mb	2017-07-17

ID:: 3561129

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