GSM8224558: mouse kidney_sham_24h_4; Mus musculus; RNA-Seq - SRA

SRX24344733: GSM8224558: mouse kidney_sham_24h_4; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 8.1M spots, 946.7M bases, 249.6Mb downloads

External Id: GSM8224558_r1

Submitted by: Institute of Translational Physiology, Charité - Universitätsmedizin Berlin

Study: Transcriptomic analysis of rhabdomyolysis induced kidney injury

PRJNA1103748 • SRP503561 • All experiments • All runs

show Abstracthide Abstract

Acute kidney injury (AKI) represents a syndrome seldom characterized by a single, distinct pathophysiological cause. Rhabdomyolysis-induced acute kidney injury (RIAKI) constitutes roughly 15% of AKI cases, yet its underlying pathophysiology remains poorly understood. Using a murine model of RIAKI induced by muscular glycerol injection, we aimed to analyse transcriptomic alterations by RNAseq. Overall design: We analysed kidneys lysates following 24 hours of AKI induction by rhabdomyolysis (N=6) to obtain a transcriptomic profile at a stage of beginning kidney failure (significant raise of creatinine).

Sample: mouse kidney_sham_24h_4

SAMN41059476 • SRS21113090 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM8224558

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA extraction from frozen kidney samples was performed using RNA-STAT-60 (Tel-Test, Inc. USA) according to the manufacturer's instructions followed by cDNA synthesis with random primers and Superscript II reverse transcriptase (Thermo Fisher Scientific Inc., USA). QIAseq UPX 3' Transcriptome Kit (QIAGEN) according to the manufacturers instruction

Runs: 1 run, 8.1M spots, 946.7M bases, 249.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR28780080	8,114,184	946.7M	249.6Mb	2024-09-18

ID:: 32658804

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