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SRX2422601: GSM2428818: TETS_ChIP-seq_H3K4me3_stage9_replicate2; Xenopus tropicalis; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 26.2M spots, 2G bases, 835Mb downloads

Submitted by: NCBI (GEO)
Study: Regulatory remodeling in the allo-tetraploid frog Xenopus laevis
show Abstracthide Abstract
Genome duplication has played a pivotal role in the evolution of many eukaryotic lineages, including the vertebrates.The most recent vertebrate genome duplication is that in Xenopus laevis, which resulted from the hybridization of two closely related species about 17 million years ago. Here we generated epigenetic profiles and determined gene expression in X.laevis embryos to study the consequences of this duplication at the level of the genome, the epigenome and gene expression. Overall design: Genomic DNA of Xenopus tropicalis and Xenopus laevis; RNA-seq (two replicates) in stage 10.5 Xenopus laevis embryos; ChIP-seq for H3K4me3 and p300 (each two replicates) in Xenopus laevis (LELS), Xenopus tropicalis (TETS) and Xenopus tropicals x laevis (hybrid; LETS) embryos.
Sample: TETS_ChIP-seq_H3K4me3_stage9_replicate2
SAMN06141419 • SRS1859704 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Protein-DNA complexes were isolated with antibody from whole embryo lysates that were fixed, sonicated and yolk depleted. Libraries were prepared with the Kapa Hyper Prep kit (Kapa Biosystems)
Experiment attributes:
GEO Accession: GSM2428818
Links:
Runs: 1 run, 26.2M spots, 2G bases, 835Mb
Run# of Spots# of BasesSizePublished
SRR511021526,206,7532G835Mb2017-10-11

ID:
3518324

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