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SRX2418128: GSM2425844: Salmonella/H.poly Cell 17; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 973,421 spots, 74M bases, 27.6Mb downloads

Submitted by: NCBI (GEO)
Study: A single-cell survey of the small intestinal epithelium
show Abstracthide Abstract
To understand the diversity of cellular states within mouse intestinal epithelial tissue, we obtained whole intestines from wild type mice, dissagregated the samples, sorted into single cells and profiled them by single-cell RNA-seq. Overall design: To understand normal tissue homeostasis, untreated cells were profiled, while to investiage the impact of enteric pathogens on epithelial cells, mice infected with both Salmonella Enterica and the parasitic worm H. polygyrus were profiled using both 3'-droplet-based and full length plate-based single-cell RNAseq. RANKL-mediated differentiation of organoid cultures were used to sequence the rare intestinal M cell, these were then compared to M cells obtained from the in vivo follicle associated epithelium (FAE). Please note that the FAE_UMIcounts.txt file has been updated on May 7th 2020.
Sample: Salmonella/H.poly Cell 17
SAMN06139923 • SRS1855450 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: 3' polyA RNA seq 10X Genomics Chromium Prep Full length RNAseq (SMART-Seq2)
Experiment attributes:
GEO Accession: GSM2425844
Links:
Runs: 1 run, 973,421 spots, 74M bases, 27.6Mb
Run# of Spots# of BasesSizePublished
SRR5101968973,42174M27.6Mb2017-11-12

ID:
3513737

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