GSM8150437: CB-D4_cDC1_pool_S19; Homo sapiens; RNA-Seq - SRA

SRX23964743: GSM8150437: CB-D4_cDC1_pool_S19; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 2000) run: 19M spots, 1.9G bases, 975.6Mb downloads

External Id: GSM8150437_r1

Submitted by: The University of Melbourne

Study: Gene expression profile of in-vitro differentiated Dendritic Cells (DCs) from induced pluripotent stem cell (iPSC) and cord blood sources

PRJNA1088507 • SRP495509 • All experiments • All runs

show Abstracthide Abstract

Dendritic cells (DCs) are rare innate immune cells that are essential regulators of anti-tumour, anti-viral and vaccine responses by the adaptive immune system. We established a protocol to differentiate human dendritic cells from iPSCs and compare their behaviour with cord blood_derived equivalents. We used bulk RNA-seq analysis to characterise the different subsets of in vitro-generated DCs including DC1, DC2A and DC2B. Overall design: In vitro-differentiated DC subsets from iPSC and cord blood CD34+ progenitors were isolated by Fluorescence-activated cell sorting (FACS) as Live HLADR+ CD11c+ CD141+ CLEC9A+ DC1, HLADR+ CD11c+ CD1c+ CD14- DC2A and HLADR+ CD11c+ CD1c+ CD14+ DC2B subsets and were analysed by bulk RNA sequencing.

Sample: CB-D4_cDC1_pool_S19

SAMN40475391 • SRS20764381 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM8150437

Instrument: NextSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was harvested using Qiagen RNeasy® Plus micro Kit (Qiagen, cat#74034) Multiplex RNA-Seq libraries were processed starting with 10ng of total RNA. The libraries were made by individual first strand synthesis to add the i7 index sequences. Samples were then pooled for subsequent processing.

Runs: 1 run, 19M spots, 1.9G bases, 975.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR28359589	19,029,280	1.9G	975.6Mb	2024-04-26

ID:: 32270673

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