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SRX23331153: GSM8027429: SJAML079769_C2-Molm13_KO-H2AK119ub1-AB1; Homo sapiens; OTHER
1 ILLUMINA (Illumina NovaSeq 6000) run: 28.7M spots, 5.4G bases, 812.4Mb downloads

External Id: GSM8027429_r1
Submitted by: ST JUDE CHILDREN'S RESEARCH HOSPITAL
Study: Functional Characterization of Cooperating MGA Mutations in RUNX1::RUNX1T1 Acute Myeloid Leukemia [Cut&Run]
show Abstracthide Abstract
MGA (Max-gene associated) is a dual-specificity transcription factor that negatively regulates MYC-target genes to inhibit proliferation and promote differentiation. Loss-of-function mutations in MGA have been commonly identified in several hematological neoplasms, including acute myeloid leukemia (AML) with RUNX1::RUNX1T1, however, very little is known about the impact of these MGA alterations on normal hematopoiesis or disease progression. We show that representative MGA mutations identified in patient samples abolish protein-protein interactions and transcriptional activity. Using a series of human and mouse model systems, including a newly developed conditional knock-out mouse strain, we demonstrate that loss of MGA results in upregulation of MYC and E2F targets, cell cycle genes, mTOR signaling, and oxidative phosphorylation in normal hematopoietic cells, leading to enhanced proliferation. The loss of MGA induces an open chromatin state at promotors of genes involved in cell cycle and proliferation. RUNX1::RUNX1T1 expression in Mga-deficient murine hematopoietic cells leads to a more aggressive AML with a significantly shortened latency. These data show that MGA regulates multiple pro-proliferative pathways in hematopoietic cells and cooperates with the RUNX1::RUNX1T1 fusion oncoprotein to enhance leukemogenesis. Overall design: Cut&Run genomic occupancy analysis of human (Molm13) or mouse (HSCs) with deletions of MGA
Sample: SJAML079769_C2-Molm13_KO-H2AK119ub1-AB1
SAMN39510302 • SRS20193606 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: GSM8027429
Instrument: Illumina NovaSeq 6000
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: CUT&RUN was performed following the kit from EpiCypher (cat# 14-1048) CUT&RUN DNA libraries were made following NEBNext Ultra II DNA Library Prep Kit for Illumina (cat# E7103), PCR amplification was done for 12 cycles
Runs: 1 run, 28.7M spots, 5.4G bases, 812.4Mb
Run# of Spots# of BasesSizePublished
SRR2766315228,672,6715.4G812.4Mb2024-05-15

ID:
31542992

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