SRX23314870: Nano3-seq of C. elegans pup mutants, whole animal, hermaphrodite
1 OXFORD_NANOPORE (MinION) run: 84,293 spots, 56.8M bases, 50.7Mb downloads
Design: Total RNA was isolated from 1-day old adult hermaphrodite wildtype and mutant C. elegans raised at 22C; total RNA was rRNA-depleted via an RNase H method; libraries were generated following the Nano3P-seq technique (modification of direct cDNA-seq) wherein a DNA-RNA hybrid oligo captured RNA ending in any nucleotide.
Submitted by: Syracuse University
Study:
Sequencing experiments on poly(U) polymerase mutants in C. elegansshow Abstracthide AbstractSequenced small RNA (Illumina) and mRNA (Nanopore) for 1-day old adult C. elegans (whole tissue) of various poly(U) polymerase deletion mutants.
Library:
Name: pup3_mRNA_rep2
Instrument: MinION
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Runs:
1 run, 84,293 spots, 56.8M bases, 50.7Mb