Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNAs of were isolated by Trizol reagent (Invitrogen), and were treated with DNase I (Ambion) to remove DNA. mRNAs were enriched with Oligo (dT)-conjugated magnetic beads and were then sent to the Genomics Core Facilities of the Shanghai Center for Plant Stress Biology, SIBS, CAS (Shanghai, China) for library preparation and Illumina sequencing.