U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX22993319: GSM7987610: APOE-/- sham mice + PBS; Mus musculus; RNA-Seq
2 ILLUMINA (Illumina NovaSeq 6000) runs: 631M spots, 122.3G bases, 37.2Gb downloads

External Id: GSM7987610_r1
Submitted by: China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University
Study: Molecular characteristics of microglia in APOE-/-mice treated with APOE130-149 or control peptides after NMOSD induction
show Abstracthide Abstract
NMOSD is an autoimmune astrocytopathy of the central nervous system, mediated by antibodies against aquaporin-4 water channel protein (AQP4-Abs), resulting in damage of astrocytes with subsequent demyelination and axonal damage. The extracellular communication through astrocyte-derived extracellular vesicles (ADEVs) has received a growing interest in association with astrocytopathies, however, to which extent ADEVs contribute to NMOSD pathogenesis remains unclear. Here, we demonstrate a significant increase in apolipoprotein E (APOE)-rich ADEVs in AQP4-Abs-positive NMOSD patients as well as in a murine model of NMOSD.To capture further insight regarding the effects of APOE on microglial functional status, scRNA-seq was performed to identify molecular characteristics of microglia of APOE-/- mice subjected to sham or NMOSD induction and treated with APOE130-149 or control peptides. We demonstrated that APOE130-149 treatment has a substantial effect on microglial transcriptomes and biological features, suppressing molecules associated with microglia activation and pro-inflammatory functions. Overall design: To capture insight regarding the effects of APOE on microglial functional status, scRNA-seq was performed to identify molecular characteristics of microglial cells of APOE-/- mice subjected to sham or NMOSD induction and treated with APOE130-149 or control peptides. CD45intCD11b+ cells were sorted from the mouse brain by flow cytometry on day 4 after NMOSD induction, and were subjected to single cell RNA sequencing. Data were pooled from 4 mice each group.
Sample: APOE-/- sham mice + PBS
SAMN39049360 • SRS19957990 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM7987610
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Single-cell suspensions were prepared from pooled mouse brain tissues at day 4 after sham or NMOSD induction. CD45intCD11b+ cells were isolated at 4°C using flow-cytometry. Library was performed according to the manufacter's instructions (single-cell 3' v.2 kit, 10× Genomics).
Runs: 2 runs, 631M spots, 122.3G bases, 37.2Gb
Run# of Spots# of BasesSizePublished
SRR27316058131,902,58725.5G7.7Gb2024-01-30
SRR27316059499,129,42996.8G29.5Gb2024-01-30

ID:
31066768

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...