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SRX22819441: GSM7951841: BM_2_BCR; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 17.9M spots, 5.4G bases, 1.7Gb downloads

External Id: GSM7951841_r1
Submitted by: Fudan University
Study: A biphenotypic lymphocyte subset displays both T- and B-cell functionalities
show Abstracthide Abstract
T cell/B cell mixed phenotypic lymphocytes have been observed in different disease contexts, yet their presence and function in physiological conditions remain elusive. Here, we provide evidence for the existence of a lymphocyte subset endogenously expressing both T- and B-cell lineage markers in mice by single cell RNA sequencing. Overall design: Bone marrow, lymph node and peripheral blood cells isolated from C57BL/6J female mice were stained with Live/Dead dye and lymphocytes were isolated using a BDFACSAria™ IIISorter. Cells were manually counted by Trypan blue and AO-PI after each centrifugation and resuspension. Single cells were processed using Chromium Controller (10x Genomics) according to the manufacturer's protocol. By using Chromium Next GEM Single Cell 5' Kit v2 and Chromium Next GEM Chip K Single Cell Kit , we performed single cell TCR/BCR-seq and 5' gene expression profiling.
Sample: BM_2_BCR
SAMN38718431 • SRS19800991 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM7951841
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC SINGLE CELL
Selection: cDNA
Layout: PAIRED
Construction protocol: Bone marrow, lymph node and peripheral blood cells isolated from C57BL/6J female mice were stained with Live/Dead dye and lymphocytes were isolated using a BDFACSAria™ IIISorter. Cells were manually counted by Trypan blue and AO-PI after each centrifugation and resuspension. By using Chromium Next GEM Single Cell 5' Kit v2 (10x Genomics, 1000263) and Chromium Next GEM Chip K Single Cell Kit (10x Genomics, 1000287), we performed single cell TCR/BCR-seq and 5' gene expression profiling. The cell suspension was loaded onto the Chromium single cell controller (10x Genomics) to generate single-cell gel beads in the emulsion according to the manufacturer's protocol. Captured cells were lysed and the released RNA were barcoded through reverse transcription in individual GEMs. Cell-barcoded 5' gene expression libraries and V(D)J enriched TCR/BCR libraries were sequenced on an Illumina NovaSeq6000 system.
Runs: 1 run, 17.9M spots, 5.4G bases, 1.7Gb
Run# of Spots# of BasesSizePublished
SRR2713761217,928,4455.4G1.7Gb2024-01-01

ID:
30861386

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