GSM7916683: monolayer cultured hepatocytes for 7 days, replicate 3; M... - SRA

SRX22635358: GSM7916683: monolayer cultured hepatocytes for 7 days, replicate 3; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 9.4M spots, 328.4M bases, 173.5Mb downloads

External Id: GSM7916683_r1

Submitted by: Division of Organogenesis and Regeneration, Medical Institute of Bioregulation, Kyushu University

Study: Hepatocytes differentiate into intestinal epithelial cells through a hybrid epithelial/mesenchymal cell state in culture II

PRJNA1044777 • SRP474011 • All experiments • All runs

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We found that hepatocytes isolated from adult mouse livers can dedifferentiate into progenitor-like cells and exhibit a hybrid epithelial/mesenchymal phenotype in monolayer culture. dediHeps can re-differentiate into mature hepatocytes by forming aggregates and fetal intestinal progenitor cells (FIPCs) by forming organoids in three-dimensional culture. We conducted RNA-seq analyses to investigate the characteristincs of hepatocytes, dediHeps, dediHep aggregates, dediHep derived spherical organoids, dediHep derived budding organoids, FIPC derived spherical organoids, intestinal stem cell (ISC) derived budding organoids, and CLiP cells (Katsuda, 2016). Overall design: We performed RNA-seq analyses for hepatocytes, dediHeps, dediHep aggregates, dediHep-derived spherical organoids, dediHep-derived budding organoids, FIPC-derived spherical organoids, ISC-derived budding organoids, and CLiPs.

Sample: monolayer cultured hepatocytes for 7 days, replicate 3

SAMN38411943 • SRS19633152 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM7916683

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was prepared from hepatocytes, dediHeps, dediHep aggregates, dediHep-derived organoids, FIPC-derived organoids, ISC-derived organoids, and CLiPs using an ISOGEN II (Nippon gene, Tokyo, Japan) or RNeasy mini kit (QIAGEN, Venlo, Netherlands), according to the manufacturer's instructions. Detailed protocol is described in "CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq (PMID: 27121950)".

Runs: 1 run, 9.4M spots, 328.4M bases, 173.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR26941570	9,382,850	328.4M	173.5Mb	2024-04-02

ID:: 30643775

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