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SRX22167725: GSM7852960: GC-2 cells, DMSO, normoxia 24h, 3 [ND3]; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 21.7M spots, 6.5G bases, 2Gb downloads

External Id: GSM7852960_r1
Submitted by: Wuhan University
Study: Inhibiting neddylation with MLN4924 potentiates hypoxia-induced apoptosis of mouse type B spermatogonia GC-2 cells
show Abstracthide Abstract
Hypoxia, an inadequate supply of tissue oxygen tension, has been reported to induce apoptosis of spermatogenic cells and is associated with male infertility. Neddylation, a post-translational modification similar to ubiquitination, has been shown to be involved in the hypoxia stress response. However, the functions of neddylation in hypoxia-induced apoptosis of spermatogenic cells and its association with male infertility remain largely unexplored. In this study, aiming to explore the role of neddylation in male infertility, we used the specific neddylation inhibitor MLN4924 for treatment in mouse type B spermatogonia GC-2 cells. Our results showed that MLN4924 had no apparent effect on GC-2 cell apoptosis under normoxia, but significantly increased apoptotic cells under hypoxia. Transcriptomic analysis and qPCR assay confirmed that MLN4924 could suppress the expression of hypoxia target genes in GC-2 cells under hypoxia. In addition, MLN4924 could enhance the induction of intracellular and mitochondrial reactive oxygen species (ROS) under hypoxia. These results indicate that the neddylation inhibitor MLN4924 potentiates hypoxia-induced apoptosis of mouse type B spermatogonia GC-2 cells, and neddylation may play an important role in promoting spermatogenic cells to adapt to hypoxia stress. Overall design: GC-2 cells were incubated with MLN4924 (1 µM) or DMSO as control, and then the cells were cultured under normoxia or hypoxia for 24 hours (each sample with 3 biological replicates).
Sample: GC-2 cells, DMSO, normoxia 24h, 3 [ND3]
SAMN37920523 • SRS19225590 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM7852960
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNAs were extracted using RNAiso Plus (TaKaRa Bio),and then purified using the RNeasy Mini Kit (QIAGEN, 74104). Sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, #E7530L) according to the manufacturer's protocol. Qualified libraries were pooled and sequenced on Illumina NovaSeq platforms using PE150 strategy at Wuhan Benagen Technology Co., Ltd. (Wuhan, China).
Runs: 1 run, 21.7M spots, 6.5G bases, 2Gb
Run# of Spots# of BasesSizePublished
SRR2646372621,695,6096.5G2Gb2023-11-02

ID:
30122449

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