GSM7794405: KO_7.6_DMSO_6h_2; Mus musculus; RNA-Seq - SRA

SRX21842253: GSM7794405: KO_7.6_DMSO_6h_2; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 5.1M spots, 384.6M bases, 169.6Mb downloads

External Id: GSM7794405_r1

Submitted by: Xavier Lab, Broad Institute of MIT and Harvard

Study: Small molecule probe for IBD risk variant GPR65 I231L alters cytokine signaling networks through positive allosteric modulation

PRJNA1019398 • SRP462096 • All experiments • All runs

show Abstracthide Abstract

BRD5075 is a positive allosteric modulator of GPR65. Activity has been demonstrated by cAMP production in HeLa cells overexpessing GPR65 and target engagment has been confirmed. BRD5075 also decreases TNF-a secretion by GPR65 WT BMDCs. Goal is to identify differentially expressed genes in response to this GPR65 PAM Overall design: Total 90 samples. GPR65 WT, variant I231L, and KO BMDCs stimulated with different pH and GPR65 PAM in triplicate on the same plate for each timepoint (2 hours and 6 hours). Positive control is PGE2, negative is DMSO.

Sample: KO_7.6_DMSO_6h_2

SAMN37484081 • SRS18940340 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM7794405

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Centrifuge plates at 100xg for 3 mins, remove supernatant. Wash 1X with 100uL PBS, aspirate. Add 100uL lysis buffer, pipet up and down and freeze at -80C. Libraries were constructed using a modified SmartSeq2 protocol.RNA lysate were purified with Dynabeads mRNA Direct Purification kit (Life Technologies),followed by reverse transcription with Maxima Reverse Transcriptase (Life Technologies) and whole transcription amplification (WTA) with KAPA HotStart HiFi 2x ReadyMix (Kapa Biosystems).WTA products were purified with Ampure XP beads (Beckman Coulter). RNA-Seq libraries were constructed using Nextera XT DNA Library Preparation kit (Illumina). Libraries were sequenced with Nextseq500(75cycles) paired-end and the following parameters : paired End: Index1 8, Index2 8, Read1 38, Read2 38

Runs: 1 run, 5.1M spots, 384.6M bases, 169.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR26128705	5,061,116	384.6M	169.6Mb	2024-06-27

ID:: 29661024

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity