GSM2320010: Mon-24h-siPU Rep1 RNA-seq; Homo sapiens; RNA-Seq - SRA

SRX2174268: GSM2320010: Mon-24h-siPU Rep1 RNA-seq; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 13.5M spots, 1.1G bases, 474Mb downloads

Submitted by: NCBI (GEO)

Study: Dynamic gene regulatory networks of human myeloid differentiation [RNA-seq_siRNA]

PRJNA343380 • SRP090090 • All experiments • All runs

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We utilize gene expression and open chromatin footprinting data to build a gene regulatory network of key transcription factors that capture the cell and time-specific regulatory programs specified during human myeloid differentiation. Overall design: RNA-seq profiling of undifferentiated HL-60, differentiating macrophage, neutrophil, monocyte, following siRNA control and siPU.1 treatment for 24 hours.

Sample: Mon-24h-siPU Rep1 RNA-seq

SAMN05786735 • SRS1701604 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA quality was evaluated for all samples using an Agilent 2100 Bioanalyzer. Samples with a RIN > 9 were used to build RNA-seq libraries from 50,000 cells using the Smart-seq2 protocol (Picelli et al., 2013). Reads were sequenced as 40 bp paired-end dual indexed reads using Illumina’s NextSeq 500

Experiment attributes:

GEO Accession: GSM2320010

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 13.5M spots, 1.1G bases, 474Mb

Run	# of Spots	# of Bases	Size	Published
SRR4254386	13,543,255	1.1G	474Mb	2017-04-06

ID:: 3169194

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