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SRX21481432: GSM7733662: HEK293,polyA,rep1; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 23.9M spots, 7.2G bases, 4.6Gb downloads

External Id: GSM7733662_r1
Submitted by: Ge Shan, University of Science and Technology of China
Study: Systematic identification and characterization of Exon-intron circRNAs (RNA-Seq)
show Abstracthide Abstract
Exon-intron circRNA (EIciRNA) is a subclass of backsplicing-generated circRNAs featured with intron retention, among which some play roles in transcriptional regulation. We aim to characterize EIciRNAs by developing pipeline and investigate the factors involved in regulating EIciRNA biogenesis using CRISPR-Cas9 screen, as well as the physiology functions of EIciRNAs during neuronal differentiation. Overall design: To detect EIciRNAs and intron retention in the human transcriptome, we performed both RNase R-treated and poly(A)+ RNA-seq in HEK293 cells. To investigate the regulation of SRSF1 on EIciRNA biogenesis, SRSF1 knockdown cell line was established and both the two types of RNA-seq were performed. To investigate the functional roles of EIciRNAs during neuronal differentiation, differentiated neuronal cell line was established by inducing SH-SY5Y cells using Retinoic acid (RA).
Sample: HEK293,polyA,rep1
SAMN37146999 • SRS18714017 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: GSM7733662
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted by Trizol reagent (Invitrogene), according to the standard protocol. The ribosome RNA (rRNA) was removed by Ribominus kit. RNA libraries were prepared for sequencing using standard Illumina protocols.
Runs: 1 run, 23.9M spots, 7.2G bases, 4.6Gb
Run# of Spots# of BasesSizePublished
SRR2575851023,937,6947.2G4.6Gb2024-03-04

ID:
28922763

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