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SRX21431684: GSM7720021: B. cereus, LF10, Rep a; Bacillus cereus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 30M spots, 9.1G bases, 2.7Gb downloads

External Id: GSM7720021_r1
Submitted by: Institute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation
Study: Transcriptome analysis of Bacillus cereus strain INRA C3 in response to iron depletion and lactoferrin, accomplished by RNA sequencing
show Abstracthide Abstract
The iron-binding glycoprotein lactoferrin exhibits anti-bacterial activity. To investigate the overall response of Bacillus cereus to lactoferrin, total transcriptome analyses were performed. Growth conditions were mMOD minimal medium, minimal medium plus 10 mg/ml lactoferrin, as well as minimal medium without iron source for comparison. Differential regulation of genes involved in iron acquisition as well as genes encoding enterotoxins and further virulence factors was of special interest. Overall design: For subsequent RNA sequencing, strain INRA C3 was grown for two hours in mMOD minimal medium, mMOD-Fe (without iron source) and mMOD supplemetned with 10 mg/ml lactoferrin. Two biological replicates were prepared per growth condition.
Sample: B. cereus, LF10, Rep a
SAMN37073796 • SRS18669108 • All experiments • All runs
Organism: Bacillus cereus
Library:
Name: GSM7720021
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA preparation and on-column DNase digestion were performed using RNeasy® Mini Kit (Qiagen) according to the instructions of the manufacturer. RNA quality was tested via spectrophotometer and agarose gel electrophoresis. RNA purity was confirmed via PCR for 16 S rRNA. Random primed cDNA libraries were constructed according to the Illumina Genome Analyzer II protocol.
Runs: 1 run, 30M spots, 9.1G bases, 2.7Gb
Run# of Spots# of BasesSizePublished
SRR2570680329,994,8269.1G2.7Gb2023-10-01

ID:
28871513

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