GSM7706729: Microbiota of Saliva from subject RCC_8; Homo sapiens; OT... - SRA

SRX21337117: GSM7706729: Microbiota of Saliva from subject RCC_8; Homo sapiens; OTHER
1 ILLUMINA (Illumina MiSeq) run: 86,331 spots, 51.9M bases, 30.2Mb downloads

External Id: GSM7706729_r1

Submitted by: Experimental and Clinical Biomedical Sciences, University of Florence

Study: First exploration of the altered microbial gut-lung axis in the pathogenesis of human refractory chronic cough

PRJNA1004563 • SRP454629 • All experiments • All runs

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v3-v4 16S rRNA sequencing was used to characterize both gut and oral microbiota composition of RCC (refractory chronic cough) patients and matched healthy controls (HC). The groups are matched in age and gender. Overall design: Total DNA was extracted from stool and saliva of 10 patients and 10 healthy subjects, amplified to collect amplicons of variable V3–V4 regions (primers 341F and 805R) of the bacterial 16s rRNA gene and sequenced with MiSeq (2x300bp) Illumina platform.

Sample: Microbiota of Saliva from subject RCC_8

SAMN36944000 • SRS18582360 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM7706729

Instrument: Illumina MiSeq

Strategy: OTHER

Source: GENOMIC

Selection: other

Layout: PAIRED

Construction protocol: Genomic DNA was extracted using the DNeasy PowerSoil Pro Kit (Qiagen, Hilden, Germany) Illumina 16S Metagenomic Sequencing Library Preparation protocol ( see https://emea.support.illumina.com)

Runs: 1 run, 86,331 spots, 51.9M bases, 30.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR25609990	86,331	51.9M	30.2Mb	2024-04-17

ID:: 28774568

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